Comparative Analysis of Grapevine Epiphytic Microbiomes among Different Varieties, Tissues, and Developmental Stages in the Same Terroir

There is limited knowledge about the relationships of epiphytic microbiomes associated with phyllosphere different Vitis vinifera cultivars in same vineyard and terroir. To address this research gap, we investigated microbiome compositionof 36 grapevine genotypes grown plant sections during growing season. Using high-throughput NGS-based metagenomic analysis targeting ITS2 V4 regions 16S ribosomal gene fungal bacterial communities, respectively, weassessed impact on microbial assemblages various parts phyllosphere. The results indicated that tissues display high diversity regardless cultivars’ identity use. selected three representing distinct phenological stages, namely bark bud, berry set, fruit harvest, had almost a similar number OTUs, while difference was recorded for species. harvest stage hosted highest whereas bud contained lower. Bacterial dominant phyla were Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, genera Gluconacetobacter, Erwinia, Gluconobacter, Zymobacter, Buchnera, Pseudomonas, Pantoea, Hymenobacter, Pedobacter, Frigoribacterium, Sphingomonas, Massilia. For fungi, Ascomycota Basidiomycota, Aureobasidium, Cladosporium, Alternaria, Aspergillus, Davidiella, Phoma, Epicoccum, Rhodosporidium, Glomerella, Botryosphaeria, Metschnikowia, Issatchenkia, Lewia. Both genotype cultivar appeared to considerably shape structure within Taken together, these indicate could be proved an important molecular fingerprint provide efficient management tool traceability wine grape end products. Moreover, unique signatures highlights need further development precision support viticulture sustainability face climate change.